pcna  (Bioss)

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) Schematic of a sagittal view of the zebrafish brain highlighting the proximate location of the diencephalic posterior tubercular nucleus (PTN) and innervation of the Mauthner cells (M-cell). (B) Confocal projections of PCNA expression in a representative communal, dominant, and subordinate Tg( dat :egfp) zebrafish. Merged channel also shows DAPI nuclear staining in blue. Arrowheads point to PCNA expressing PTN somata. Scale bar = 20 μm. (C) Summary graph of the fraction of PTN neurons that express PCNA. Symbols represent individual samples; bars represent averages, error bars represent SEM. Dashed lines show pairwise comparison connecting each dominant to its subordinate counterpart. (Com n=6, Dom n= 8, Sub n= 8; One-way ANOVA, P=0.0029 , One-way ANOVA, Tukey’s Multiple Comparison post-test).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques: Expressing, Staining, Comparison

Journal: bioRxiv

Article Title: Cellular mechanisms underlying social regulation of the posterior tubercular nucleus in zebrafish ( Danio rerio )

doi: 10.64898/2026.01.27.701991

Figure Lengend Snippet: (A) PC1 and PC2 are the first and the second principal components excluding social isolates. (B) Eigenvalues of the principal components excluding social isolates showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. (C) PC1 and PC2 are the first and the second Principal components excluding PCNA as a component. (D) Eigenvalues of the principal components excluding PNCA as a component showing the percentage of variation explained by each component. Line plot illustrates the cumulative explained variance. In A and C, points represent individual samples (n= 6 per social condition), different colors and symbols represent different groups. Ellipses represent 68% confidence intervals of core regions. Loading vectors represent original variables, the directions of arrows represent correlation between original variable and principal components, lengths represent association of original data to principal components. In B and D components are listed in descending order (highest to the lowest).

Article Snippet: Another series of PBS washes (3-4 times for 5 minutes each) was performed, and slices were stained with a rabbit polyclonal anti-PCNA primary antibody (Cell Signaling, 2586t) at a concentration of 1:500.

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: 131 I/MTX-loaded engineered outer membrane vesicles for sequentially coordinated radio-metabolic-immunotherapy of glioma

doi: 10.1186/s12951-025-03936-4

Figure Lengend Snippet: 131 I-O/M-P inhibits tumor cell-cycle and metabolism to enhance radio-sensitivity in vitro. ( A ) Relative cell viability of HUVEC and GL261 cells after incubation with different concentrations of OMVs for 72 h. Error bars represent mean ± s.d. ( n = 6). ( B-C ) Cellular uptake of GL261 cells. Flow cytometry results ( B ) and corresponding DiI fluorescence intensities ( C ) of GL261 cells treated with DiI-O/M-P at different time points. Error bars represent mean ± s.d. ( n = 3). ( D ) Confocal laser scanning microscope of GL261 cells after 12 h treatment with DiI-O/M-P (Scale bar = 100 μm). (E) Flow cytometry was performed to assess the cell cycle in GL261 cells treated with MTX and O/M-P for 24 h. ( F ) The proportion of cells in G0/G1, S and G2/M phases. Error bars represent mean ± s.d. ( n = 3). ( G-H ) Western blot analysis of cell cycle-related proteins, including cyclin A2 and PCNA. ( H ) Densitometry quantification of western blot. Error bars represent mean ± s.d. ( n = 3). ( I-J ) The expression of dihydrofolate reductase (DHFR) in GL261 cells treated with MTX and O/M-P for 24 h was analyzed by western blot. Error bars represent mean ± s.d. ( n = 3). ( K-L ) Western blot analysis of cGAS-STING related proteins, including STING, p-STING, IRF3 and p-IRF3 from treated GL261 cells. Error bars represent mean ± s.d. ( n = 3). ( M ) GL261 cell viability after 24 h incubation with O/M-P, 131 I, 131 I-O-P, and 131 I-O/M-P. Error bars represent mean ± s.d. ( n = 6). ( N-O ) Confocal immunofluorescence images ( N ) and corresponding fluorescence intensities ( O ) of γ-H2AX in GL261 cells after different treatments (Scale bar = 100 μm). Error bars represent mean ± s.d. ( n = 3). ( P-Q ) FACS plots ( P ) and quantitative analysis ( Q ) of apoptosis in GL261 cells after different treatments

Article Snippet: The PVDF membrane was incubated with primary antibodies cyclin A2 Polyclonal antibody, Proteintech; PCNA Polyclonal antibody, Proteintech) overnight at 4 °C.

Techniques: In Vitro, Incubation, Flow Cytometry, Fluorescence, Laser-Scanning Microscopy, Western Blot, Expressing, Immunofluorescence

Journal: Journal of Nanobiotechnology

Article Title: 131 I/MTX-loaded engineered outer membrane vesicles for sequentially coordinated radio-metabolic-immunotherapy of glioma

doi: 10.1186/s12951-025-03936-4

Figure Lengend Snippet: 131 I-O/M-P treatment of GL261 subcutaneous tumors. ( A ) Schematic illustration showing the experimental design of treatments for GL261 subcutaneous tumor-bearing mice. ( B ) Individual mouse tumor volume changes after different treatments. ( C-F ) Immunofluorescence staining ( C , E ) and the quantified analysis ( D , F ) of tumors tissues collected from GL261 tumor-bearing mice after different treatments (Scale bar = 100 μm). C-D: Ki67; E-F: PCNA. ( G-J ) Representative flow cytometry plots ( G , I ) and the quantified analysis ( H , J ) showing different types of T cells in subcutaneous tumors from different groups on day 5 after each treatment. G-H: CD3 + CD8 + Granzyme B + T cells; I-J: CD3 + CD8 + PD-1 + T cells. Error bars represent mean ± s.d. ( n = 5). ( K-L ) Representative flow cytometry plots ( K ) and the quantified analysis ( L ) showing NK cells (CD45 + NK1.1 + ) in subcutaneous tumors from different groups on day 5 after each treatment. Error bars represent mean ± s.d. ( n = 5). ( M-P ) Immunofluorescence staining ( M , O ) and the quantified analysis ( N , P ) of tumors tissues collected from GL261 tumor-bearing mice after different treatments (Scale bar = 100 μm). M-N: CD3/CD8; O-P: NK1.1. Error bars represent mean ± s.d. ( n = 3). (ns: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: The PVDF membrane was incubated with primary antibodies cyclin A2 Polyclonal antibody, Proteintech; PCNA Polyclonal antibody, Proteintech) overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Flow Cytometry

Journal: Molecular Therapy Oncology

Article Title: Targeted nanoencapsulation of tunicamycin reduces toxicity while improving its therapeutic effectiveness in pancreatic cancer cells

doi: 10.1016/j.omton.2025.201047

Figure Lengend Snippet: Representative of H&E and PCNA IHC with TM concentrations in tumor and different organs (A) H&E staining of untreated and NP TM-treated KPC-cell-derived xenograft tumor (KPC-CDX) sections. Scale bars, 50 μm. (B) PCNA immunohistochemistry in untreated and NP TM-treated KPC-CDX sections. Bar graph showing the statistical difference of PCNA in untreated and untreated samples. Data represent mean ± SD. n = 5. Scale bars, 50 μm. (C) H&E staining in different organs of KPC-CDX mice upon NP TM treatment. 40×. Scale bars, 50 μm.

Article Snippet: Anti-rabbit IgG (# 70745) was purchased from Cell Signaling, USA; anti-mouse IgG (# sc-516102) from Santa Cruz, Inc, USA; CCN1 polyclonal antibody (#26689-1-AP) and Neuropilin-1 (NRP-1) from ProteinTech, USA; and pSTAT3 polyclonal antibody (#9145) and STAT3 polyclonal antibody, peIF4E and EIF4E antibodies, and PCNA rabbit polyclonal antibody (#13110T) from Cell Signaling, Inc., USA.

Techniques: Staining, Derivative Assay, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: The CD300c antibody CL7 suppresses tumor growth by regulating the tumor microenvironment in non-small cell lung carcinoma

doi: 10.3389/fonc.2025.1698857

Figure Lengend Snippet: CL7 reduces NSCLC cancer cell proliferation. IHC images and quantitative analyses were performed using the 10 mg/kg CL7 group, which served as the representative dose for downstream experiments. Immunohistochemistry analysis for proliferating cell nuclear antigen (PCNA), a marker of cell proliferation, was performed on lung tissue sections from tumor-bearing mice (n = 6 per group). Images were acquired using a light microscope at 20× magnification. Three random fields were selected, and PCNA-positive cells were quantified using QuPath software. All data are presented as mean ± SEM. *p < 0.05 versus PBS group.

Article Snippet: The sections were then incubated overnight at 4°C with a rabbit polyclonal anti-PCNA antibody (1:200, Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunohistochemistry, Marker, Light Microscopy, Software